Abstract #4069

Diffusion MRI acquisition methods for post-mortem imaging on a 10.5 T whole-body human MRI scanner

Benjamin C. Tendler¹, Shaun Warrington², Mohamed Kotb Selim², Wenchuan Wu¹, Gregor Adriany³, Edward J. Auerbach³, Alexander Bratch³, Hamza Farooq³, Noam Harel³, Sarah Heilbronner^3,4, Saad Jbabdi¹, Steve Jungst³, Christophe Lenglet³, Steen Moeller³, Franco Pestilli^5,6, Pramod Pisharady³, Kamil Ugurbil³, Matt Waks³, Essa Yacoub³, Stamatios N. Sotiropoulos², Karla L. Miller¹, and Jan Zimmermann^3,7

¹Wellcome Centre for Integrative Neuroimaging, University of Oxford, Oxford, United Kingdom, ²Sir Peter Mansfield Imaging Centre, University of Nottingham, Nottingham, United Kingdom, ³Centre for Magnetic Resonance Research, University of Minnesota-Twin Cities, Minneapolis, MN, United States, ⁴Baylor College of Medicine, Houston, TX, United States, ⁵Indiana University, Bloomington, IN, United States, ⁶University of Texas, Austin, TX, United States, ⁷Department of Neuroscience, University of Minnesota, Minneapolis, MN, United States

Synopsis

Keywords: Diffusion Acquisition, High-Field MRI, Post-Mortem, Macaque, Diffusion-Weighted Steady-State Free Precession

Motivation: High-quality post-mortem diffusion MRI + microscopy datasets acquired in the same brain enable novel, cross-scale neuroanatomical investigations.

Goal(s): Identify a sequence that maximises SNR-efficiency and diffusion contrast in a post-mortem macaque brain using a 10.5 T whole-body human MRI scanner.

Approach: Theoretical SNR-efficiency comparisons were performed between the diffusion-weighted spin-echo (DW-SE), stimulated-echo (DW-STE) and steady-state free precession (DW-SSFP) sequence. We performed a pilot investigation using the leading method in a post-mortem macaque brain.

Results: DW-SSFP predicted the highest SNR-efficiency across the target b-value range. Acquired DW-SSFP data (0.4 mm isotropic) demonstrated high-SNR diffusivity estimates without geometric distortions, resolving multiple-fibre populations and long-range tracts.

Impact: This research investigates acquisition methods to obtain high-quality post-mortem diffusion MRI datasets using a 10.5 T whole-body human MRI scanner. Data acquired using the described methodology provide detailed neuroanatomical information, informing future brain connectivity and in vivo investigations.

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