Abstract #4662

Multi-parametric hyperpolarized imaging of renal ischemia reperfusion injury

Alexander I Zavriyev^1,2, John Nguyen^1,3, Bukola Adebesin^1,2, Molly Sheehan¹, Daniel Boehmler⁴, Jiangsheng Yu¹, Ariful Islam⁵, Zhonglin Wang⁶, Guanghui Ge⁶, Erum Hartung⁷, Matthew Levine⁶, Stephen Kadlecek¹, and Terence Gade^1,5

¹The Metabolic Discovery Center at Penn, Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, United States, ³School of Nursing, University of Pennsylvania, Philadelphia, PA, United States, ⁴Department of Cell and Molecular Biology, University of Pennsylvania, Philadelphia, PA, United States, ⁵Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States, ⁶Department of Surgery, Children’s Hospital of Philadelphia, Philadelphia, PA, United States, ⁷Division of Nephrology, Children’s Hospital of Philadelphia, Philadelphia, PA, United States

Synopsis

Keywords: Hyperpolarized MR (Non-Gas), Hyperpolarized MR (Non-Gas), Ischemia, Renal Injury, Lysine imaging, renal ischemia reperfusion injury, kidney imaging

Motivation: Renal ischemia/reperfusion injury (IRI) significantly contributes to kidney morbidity and mortality, highlighting the need for noninvasive diagnostic strategies.

Goal(s): To investigate the effects of IRI severity on kidney function using co-hyperpolarized (HP) pyruvate and urea in a female mouse model, and test 1-¹³C lysine as a novel biomarker.

Approach: Female mice underwent ischemia for 28 or 45 minutes, followed by co-HP pyruvate and urea imaging. Separately, HP lysine imaging was performed in controls.

Results: All mice exhibited decreased urea signal in the IRI kidney. The lactate-to-urea ratio differentiated between moderate and severe injuries. Lysine imaging demonstrated strong signal localized to the kidneys.

Impact: Our results suggest that co-polarized pyruvate and urea imaging could serve as a sensitive diagnostic tool to distinguish IRI severity. Additionally, the feasibility of lysine imaging suggests the potential to enhance the specificity of HP imaging for renal IRI diagnosis.

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