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Abstract #4829

Modelling HR-MAS NMR spectra of human cerebral organoids using COLMAR1D Deep Neural Networks – comparison against peak integration

Alejandra Castilla Bolanos1, Vorapin Chinchalongporn2, Rajshree Ghosh Biswas3, Colleen Bailey2, Ronald Soong3, Dawei Li4, Raphael Bruschweiler4, Andre Simpson3, Carol Schuurmans2, and Jamie Near5
1Medical Biophysics, University of Toronto, Toronto, ON, Canada, 2Sunnybrook Research Institute, Toronto, ON, Canada, 3University of Toronto, Toronto, ON, Canada, 4The Ohio State University, Columbus, OH, United States, 5Physical Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada

Synopsis

Keywords: Software Tools, Spectroscopy, Organoids

Motivation: High-resolution magic-angle spinning (HR-MAS) NMR enables the study of metabolite concentrations in human cerebral organoids (COs). However, quantification remains challenging because HR-MAS spectra require precise NMR parameters for accurate modelling.

Goal(s): Our goal is to assess the performance of a deep neural network-based deconvolution method to model CO HR-MAS spectra for metabolite quantification.

Approach: CO HR-MAS spectra were modelled using the COLMAR1D Deep Neural Network Peak Picking tool. Quantification from COLMAR1D was compared against a peak integration.

Results: 21 metabolites were quantified in cerebral organoids. A significant correlation between COLMAR1D and peak integration was observed, although COLMAR1D estimates were significantly higher.

Impact: This study presents a convenient method for modelling and quantification of HR-MAS spectra in cerebral organoids using deep deconvolution. By reliably quantifying metabolite concentrations in human brain models, our approach will enable studies to better understand human brain metabolism.

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Keywords