Keywords: Hybrid & Novel Systems Technology, Thermometry
Motivation: Intracellular temperature plays an important role in cell metabolism but there is a lack of consensus on basic thermal properties of cells and a shortage of reliable tools to measure temperature inside of living cells.
Goal(s): We seek to improve intracellular temperature measurement for study of live cell metabolism.
Approach: We developed a custom fluorescent confocal imaging system combined with a widefield imaging path to collect ODMR based temperature measurements and localize our measurements within a cell.
Results: In our experiments with NDs implanted in live cells we observed an average temperature increase of 2.5 ±1°C after addition of a mitochondrial uncoupler.
Impact: In addition to being a stepping stone to a better understanding of intracellular thermal properties, the methods developed in this work can also be applied to studying the interplay between temperature and cancer metabolism.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords