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Abstract #5060

Potential of Intranasally Delivered Exosomes Derived from Mesenchymal Stem Cells as Treatment for Ischemic Stroke: Proton and Sodium MRI at 21.1 T

Arshia Arbabian1,2, Malathy Elumalai1, and Samuel Colles Grant1,2
1Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Tallahassee, FL, United States, 2Chemical & Biomedical Engineering, Florida State University, Tallahassee, FL, United States

Synopsis

Keywords: Stroke, High-Field MRI, Stem Cell Therapy; Diffusion; Exosomes; SPIO

Motivation: Stroke treatments are hindered by inefficient delivery and patient tolerance. This study examines whether intranasal mesenchymal stem cell-derived extracellular vesicles (EV) can overcome limitations and improve outcomes.

Goal(s): The study seeks to evaluate whether intranasal EV delivery improves recovery by investigating recovered sodium homeostasis and diffusional restrictions.

Approach: Using a preclinical ischemic stroke model, EV were administered intranasally. High-resolution MRI, including spin-echo 1H EPI DWI, T2W FSE and 23Na CSI, were acquired over 21 d.

Results: The EV-treated group displayed reduced damage and sodium imbalances, with the longitudinal consistency of ADC reflecting both reduced lesion volume and necrosis indicative of maintained cellular integrity.

Impact: MRI-based evaluation of intranasal EV therapy provides a reliable approach to monitor recovery and sodium dynamics post-stroke, meeting a critical need for non-invasive, effective treatments and their assessment.

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