Correlation of Proton Transfer Ratio and Intracellular pH in Acute Ischaemia
Jokivarsi K, Grhn H, Grhn O, Kauppinen R
A.I. Virtanen Institute for Molecular Sciences, University of Kuopio
Acidification has been shown to enhance tissue damage caused by ischaemia. Proton transfer ratio (PTR) has been recently introduced as a means to determine intracellular pH (pHi). PTR was measured in transient middle cerebral artery occlusion model of rat, compared with pHi status of the brain and progression of ischaemic tissue alterations as assessed by T1rho and diffusion MRI. A strong correlation was seen between change in PTR and pHi derived from lactate concentration, as measured by 1H MRS. These results show that cellular acidification is a key factor affecting change in PTR due to ischaemia.