Editing through Multiple Bonds: Threonine Detection
Henry P, Ugurbil K, Gruetter R, Marjanska M
University of Minnesota
In in vivo 1H spectroscopy, the signal at 1.32 ppm is usually assigned to lactate. This resonance position at physiological pH is shared with threonine, which has similar J-coupling and whose coupling partner resonates at 4.24 ppm, very close to that of lactate (4.11 ppm). The close proximity of coupling partners of lactate and threonine renders the measurement of threonine with and without editing technically challenging. The aim of this study was to exploit multiple-bond editing and quantify the threonine signal in vivo.