Dynamic Metabolic Modeling of [2-13C]Acetate Metabolism in the Rat Brain<

Alexander A. Shestov¹, Dinesh K. Deelchand¹, Pierre-Gilles Henry¹

Carbon-13 MRS combined with metabolic modeling allows measurement of metabolic rates in vivo. Most 13C metabolic modeling studies have been performed using 13C-glucose as the infused substrate. Acetate, a glial-specific substrate, is an attractive alternative to glucose for the study of neuronal-glial interactions. Here we report kinetic parameters for acetate transport and utilization, as well as dynamic metabolic modeling of glutamate and glutamine 13C turnover curves obtained during 13C-acetate infusion with a two-compartment neuronal-glial model.