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Abstract #2374

Inhibitory Effect of Carbonic Anhydrase Inhibitors on the De Novo Lipogenesis. a Study with 13C-NMR Spectroscopy

Abolghasem Mohammadi1, Dieter Leibfritz1

1Institute of Organic Chemistry, University of Bremen, Bremen, Germany

Obesity is an increasingly serious problem for the health of the population. Inhibition of de novo lipogenesis (DNL) from carbohydrate in the mammalian organism is a new approach for treatment of obesity. This inhibition is based on the inhibition of carbonic anhydrases (CA) isozymes on the step of pyruvate carboxylation in mitochondria. Sulphonamide/ Sulphamate are carbonic anhydrase inhibitors (CAIs) and inhibit DNL. We examined the effect of CA inhibition on DNL in 3T3-L1 cells in the presence of various CAIs i.e., topiramate, ethoxyzolamide, and acetazolamide by means of C-NMR spectroscopy. In the presence of CAIs DNL was 25-30% reduced.