Inhibitory Effect of Carbonic Anhydrase Inhibitors on the De Novo Lipogenesis. a Study with 13C-NMR Spectroscopy

Abolghasem Mohammadi¹, Dieter Leibfritz¹

Obesity is an increasingly serious problem for the health of the population. Inhibition of de novo lipogenesis (DNL) from carbohydrate in the mammalian organism is a new approach for treatment of obesity. This inhibition is based on the inhibition of carbonic anhydrases (CA) isozymes on the step of pyruvate carboxylation in mitochondria. Sulphonamide/ Sulphamate are carbonic anhydrase inhibitors (CAIs) and inhibit DNL. We examined the effect of CA inhibition on DNL in 3T3-L1 cells in the presence of various CAIs i.e., topiramate, ethoxyzolamide, and acetazolamide by means of C-NMR spectroscopy. In the presence of CAIs DNL was 25-30% reduced.