Kayvan Keshari1,2, David Wilson2, Mark VanCriekinge2, Daniel Vigneron2, Jeffrey Macdonald1, John Kurhnaewicz2
1University of North Carolina, Chapel Hill, NC, USA; 2University of California, San Francisco, USA
Recent studies of hyperpolarized 13C labeled compounds, specifically 13C1-pyruvate, have been used to investigate metabolic processes associated with the Warburg effect. These methods probe one specific pathway, the last step of glycolysis in which pyruvate is enzymatically converted to a number of products. Each of these products is indicative of the flux through the enzyme of choice, for example lactate production demonstrates LDH flux. This study demonstrates, for the first time, the simulataneous hyperpolarization of multiple compounds as well as their injection into an NMR compatible 3D culture system. Multi-compound hyperpolarization can lead to the real time assessment of multiple fluxes simultaneously.
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