Ahmad Raza Khan1, Poonam Rana1, Shubhra Chaturvedi2, Rajendra Prasad Tripathi3, Subash Khushu1
1NMR Research Centre, Institute of Nuclear Medicine and Allied Sciences, Delhi, India; 2Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Delhi, India; 3INMAS, Delhi, India
A high resolution 1H NMR spectroscopy based metabonomic approach has been used for finding biochemical alterations in serum as induced by radiation exposure. Blood samples were collected from mice after five days of radiation dose of 3 and 5 Gy. The proton NMR spectral analysis of serum presented the elevation of Branched amino acids, alanine, propionate and creatine and decreased concentration of lactate and choline in irradiated mice. These findings indicated enhanced protein breakdown, gluconeogenesis and altered lipid metabolism. Such metabonomic signatures may help in designing protocols and novel methodologies for screening at risk populations and measuring radiation dose.
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