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Abstract #4307

NMR-Based Metabolomics of Bacterial Infections Studied in a Mouse-Model

Verena Hoerr1, Graciela Andonegui2, Lori Zbytnuik3, Paul Kubes3, Brent W. Winston3, Hans J. Vogel1

1Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada; 2Department of Critical Care Medicine, University of Calgary, Calgary, Alberta, Canada; 3Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada

The metabolic profiles of bacterial infections were studied on a mouse-model by high resolution 1H NMR spectroscopy. Serum samples of mice, infected with Staphylococcus aureus and Streptococcus pneumoniae showed significantly higher concentrations of leucine, phenylalanine, creatine and isoleucine compared to those of the healthy control group. Additionally malonate was elevated in the case of S. aureus infection while in S. pneumoniae infected mice the taurine level was increased. Analyzing the NMR spectral data by partial least squares discriminant analysis (PLS-DA) classification along bacterial infection was revealed with high accuracy.