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Abstract #1836

Gd-Labelled Polylysine as a Tracer for Convective Enhanced Delivery

Peter Andrew Hardy1, Luke H. Bradley1, Zhiming Zhang1, Don Gash1, Dan Keeley2, Brian Kramer3, Greg Schorn4

1Anatomy & Neurobiology, University of Kentucky, Lexington, KY, United States; 2Advanced Technologies and Regenerative Medicine, Rayntham, MA, United States; 3Advanced Technologies and Regenerative Medicine, Somerville, NJ, United States; 4Advanced Technologies and Regenerative Medicine, Rayntham, Massachussets, United States

Convection Enhanced Delivery is a powerful method of delivering drugs to the CNS. MR-visible tracers co-infused with drug will be useful to assess drug distribution. We tested four compounds (Magnevist and three Gd-labeled polylysines) with molecular weights between 0.5k Da and 200 kDa as potential tracers for CED. Compounds were tested in vitro to model CED parameters used to plan delivery into four rhesus monkeys. In vitro results demonstrated MW dependent CED distribution. In vivo results demonstrated distribution of the Magnevist in the putamen but little distribution of the polylysine as a result of binding and digestion of the polylysine.