Sergey Magnitsky1, Alexander Roesch2,
Stephen Pickup1, Meenhard Herlyn2, Jerry D. Glickson1
cells were labeled with iron oxide particles and allowed to proliferate.
Small iron-retaining sub-cell population with original iron concentration
has been detected after 21 days of proliferation. This sub-cell population
exhibits high tumorigenicity, self-renewal capacity and drug resistance, and
therefore fulfills a definition of tumor initiating cells. After implantation
of labeled cells into NOD/SCID mice, iron-retaining cells have been detected
by in vivo, ex vivo MRI and Prussian blue staining.