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Abstract #3274

Detection of Pentose Phosphate Pathway Flux Using Hyperpolarized [1-13C]Gluconolactone in Mouse Livers

Karlos X. Moreno1, Crystal E. Harrison1, Matthew E. Merritt1, Zoltan Kovacs1, Zengdun Shi2, Don C. Rockey2, A Dean Sherry1, Craig R. Malloy1,2

1Advanced Imaging Research Center, Univ of TX Southwestern Med Ctr, Dallas, TX, United States; 2Internal Medicine, Univ of TX Southwestern Med Ctr, Dallas, TX, United States

Pentose phosphate pathway flux was studied using hyperpolarized δ-[1-13C]gluconolactone injected into an isolated perfused mouse liver. Control livers produced a significant amount of H13CO3-, a product indicative of pentose phosphate pathway flux and [1-13C]gluconate. Hydrogen peroxide damaged livers also produced H13CO3- and [1-13C]gluconate, though the bicarbonate was at lower amounts than the control. CCl4 treated livers did not produce any observable H13CO3-, but [1-13C]gluconate was produced. These studies show that the lactone is incorporated within the hepatocyte, phosphorylated and metabolized through the pentose phosphate pathway.