Simon Hu1, Robert Bok1, Asha
Balakrishnan2, Andrei Goga2, John Kurhanewicz1,
Daniel B. Vigneron1
1Dept. of Radiology and Biomedical
Imaging, University of California, San Francisco, San Francisco, CA, United
States; 2Dept. of Medicine, Division of Hematology/Oncology,
University of California, San Francisco, San Francisco, CA, United States
Development
of hyperpolarized technology utilizing dynamic nuclear polarization has
enabled the measurement of 13C metabolism in vivo at very high SNR. The most researched agent for in vivo
applications has been [1-13C]pyruvate. In this project, the role
of cell membrane transport on the conversion of [1-13C]pyruvate to
[1-13C]lactate and [1-13C]alanine in vivo was investigated by using the monocarboxylate transporter
inhibitor α-cyano-4-hydroxy-cinnamate. Reduced hyperpolarized alanine
and lactate were detected after α-cyano-4-hydroxy-cinnamate
administration, indicating that this inhibitor approach can be used in vivo to investigate the transport
and intracellular conversion of [1-13C]pyruvate.
Hyperpolarized
Carbon-13 & Other Nuclei II
Keywords