Abstract #4176

Cellular MRI Assessment of Magnetic Fluorescent Bead Labeled Macrophage Accumulation Following High Intensity Focused Ultrasound (HIFU) Induced Damage in a Murine Model

Hilary Hancock¹, Eric M. Gold¹, Bobbi K. Lewis¹, Melissa Smith¹, Victor Frenkel¹, Joseph A. Frank^1,2

¹Radiology and Imaging Sciences, NIH, Bethesda, MD, United States; ²National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, United States

This study investigated in vivo labeling of monocytes with SPIO/fluorescent 40nm beads followed by Cellular MRI and fluorescent microscopy to determine the effects of ablative or pulsed high intensity focused ultrasound (HIFU) in a murine model. Pulsed HIFU exposures exhibited smaller regions of edema and hypointense regions, confined to superficial muscle and dermis, on T2*W images with smaller amounts of immune response within tissues compared to ablated tissues.