Fernando
Bonetto1, Mangala Srinivas1, Bettina Weigelin2,
Luis Cruz Ricondo1, Arend Heerschap3, Carl Figdor1,
I.J. de Vries1
1Tumor
Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University
Nijmegen Medical Centre, Nijmegen, Netherlands; 2Cell Biology,
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen
Medical Centre, Nijmegen, Netherlands; 3Radiology, Radboud
University Nijmegen Medical Centre, Nijmegen, Netherlands
Dendritic
cell migration is monitored and quantified by using 19F-Chemical
Shift Spectroscoypic imaging (CSI) in a novel migration assay. 3D scaffolds
specially designed to mimic biological tissue are used in this assay. The
particular layered structure of the assay allows to assess cell migration and
to perform the control experiment simultaneously. Cells were labeled with a
perfluorocarbon compound. Our results demonstrate that 19F-CSI at
7T is suitable to track cell migration in this type of opaque assays. The
migration rates obtained in this way are comparable to clinical results
suggesting that the proposed migration assays properly mimics in-vivo
conditions.
Keywords