Friedrich Wetterling1,2, Lindsay Gallagher3,
Mhairi I. Macrae4, Sven Junge5, Andrew John Fagan,
1,6
1School of Physics, Trinity College
Dublin, Dublin, Ireland; 2Computer Assisted Clinical Medicine,
University of Heidelberg, Mannheim, Germany; 3Glasgow Experimental
MRI Centre, , Division of Clinical Neuroscience, Faculty of Medicine,
University of Glasgow, Glasgow, Scotland, United Kingdom; 4Glasgow
Experimental MRI Centre, Division of Clinical Neuroscience, Faculty of
Medicine, University of Glasgow, Glasgow, Scotland, United Kingdom; 5Bruker
BioSpin GmbH, Ettlingen, Germany; 6Centre for Advanced Medical Imaging,
St. Jamess Hospital, Dublin, Ireland
The
aim of this study was to accurately quantify subtle changes in Tissue Sodium
Concentration (TSC) during the acute phase in a rodent stroke model. A double-tuned 23Na/1H
dual resonator system was developed and a 2D radial sequence optimized for
qNa-MRM (voxel sizes of 1.2l, TA = 10min).
A quantification accuracy of <10mM was achieved, which enabled the
evolution of the TSC changes to be followed in the acute phase of
stroke. TSC maps were computed and analyzed
for each of the investigated five stroke and two sham rats from 30mins up to
8h after MCAO