Helena Bertilsson1,2, May-Britt Tessem3,
Ingrid Gribbestad4, Haakon Skogseth1, Trond Viset5,
Anders Angelsen, 2,6, Jostein Halgunset1
1Dept. of Laboratory Medicine and
Children's and Women's Health, NTNU, Trondheim, Norway; 2Dept. of
Urology, St Olav University Hospital, Trondheim, Norway; 3Dept. of
Circulation and Medical Imaging , Norwegian University of Science and
Technology (NTNU), Trondheim, Norway; 4Dept. of Circulation and
Medical Imaging, Norwegian University of Science and Technology (NTNU),
Trondheim, Norway; 5Dept. of Pathology and Medical Genetics, St
Olav University Hospital, Trondheim, Norway; 6Dept. of Cancer
Research and Molecular Medicine, NTNU, Trondheim, Norway
New
research on the genetic and metabolic level of prostate cancer is important
for future disease management in diagnostics, choice of treatment and
prognosis. This study describes a highly standardized method for
snap-freezing of a whole prostate slice that is safe (without interfering
with the routine diagnostics), easy to practise, and results in tissue with
highly intact molecular content suitable for ex vivo MR spectroscopy and gene
expression of the same sample. The present harvesting method is applicable to
all prostate cancer patients and stores the snap-frozen tissue without fixatives,
leaving it available for all kinds of future research technologies.
Keywords