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Abstract #0304

Measurement of Elevated 2-Hydroxyglutaric Acid in Brain Tumors By Difference Editing At 3T In Vivo

Changho Choi1, Sandeep Ganji1, Zoltan Kovacs1, Ralph DeBerardinis1, Elizabeth Maher1

1University of Texas Southwestern Medical Center, Dallas, TX, USA

Recent mass spectroscopy studies in-vitro and ex-vivo indicated that a fraction of gliomas contain mutations of isocitrate dehydrogenase-1 and -2, causing accumulations of 2-hydroxyglutaric acid (2HG). Here, we present in vivo detection of 2HG in human brain tumors by means of difference editing at 3T. The C2-proton resonance of 2HG at 4.02 ppm was edited using a 20 ms Gaussian radio-frequency (RF) pulse for selective 180a rotation of the its coupling partners at ~1.9 ppm. In vivo measurement was conducted in various tumors. The results indicated that 2HG is elevated to 2 - 8 mM in low-grade gliomas and secondary glioblastomas.