Quantitative Assessment of Blood-Brain-Barrier Permeability by Patlak Plots After Intraperitoneally Administrated Gadolinium-DOTA

Dana Suciu Poole¹, Johannes Rolf Sikkema², Arnoldus M. van Den Maagdenberg³, Louise van Der Weerd^2,4

Dynamic contrast-enhanced magnetic resonance imaging typically uses intravenous administration of a contrast agent. In mice, however, repeated intravenous injections often cause scarring, and insertion of permanent canulae in the tail or femoral vein require an invasive procedure and are prone to infection and damage by grooming. In this study, we have developed an alternative longitudinal DCE-MRI protocol at 9.4T using intraperitoneal administration of gadolinium-DOTA. We show that Patlak plots can be calculated following intraperitoneal administration of contrast agent on mice with unilateral blood-brain barrier impairment.