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Abstract #2289

Optimal Therapeutic Hypothermia Temperature Following Perinatal Asphyxia: A Magnetic Resonance Spectroscopy Biomarker & Immunohistochemistry Study in the Newborn Piglet.

Nicola Jayne Robertson1, Stuart Faulkner1, Manigandan Chandrasekaran1, Alan Bainbridge2, David Price2, Dorottya Kelen1, Aron Kerenyi1, Sudhin Thayyil1, Elizabeth Powell1, Ernest Cady2, Gennadij Raivich1, Xavier Golay3

1Institute for Women's Health, University College London, London, United Kingdom; 2Medical Physics & Bioengineering, University College Hospitals, London, United Kingdom; 3UCL Institute of Neurology, London, United Kingdom

Although therapeutic hypothermia is safe and effective for neonatal encephalopathy, 50% infants have adverse outcomes; clinical trials are exploring cooling to deeper temperatures for longer periods. We used our validated piglet model of perinatal asphyxia to assess the effect of cooling to 35oC, 33.5oC, and 30oC on 1H MRS-based biomarkers and immunohistochemical markers of cell death. Mild cooling (33.5oC and 35oC) was neuroprotective, but moderate cooling (30oC) led to increased cell death and raised Lac/Cr in the deep grey matter but not white matter / cortex. This confirms regional differences in optimal cooling temperatures and has importance for clinical protocols.