Abstract #3146

A 1H MRS Study on Neurospheres of Cancer Stem Cells from Human Glioblastoma Multiforme Shows the Presence of Markers of Both Glial & Neuronal Morphology

Laura Guidoni¹, Lucia Ricci Vitiani², Simona di Martino³, Sveva Grande¹, Anna Maria Luciani¹, Alessandra Palma⁴, Vincenza Viti¹, Antonella Rosi¹

¹Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanit and INFN, Rome, Italy; ²Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanit, Rome, Italy; ³Scuola Superiore di Catania, University of Catania, Catania, Italy; ⁴Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanit , Rome, Italy

Glioblastoma multiforme (GBM) is the most common and aggressive type of primary brain tumor. Cancer stem cells (CSC) refractory to the usual therapies has been demonstrated for maintenance of a subset of self-renewing cells producing cancer recurrence. We here propose ¹H MRS to study the metabolism in two CSC lines from primary GBM. The CSCs grown as neurospheres are characterised and compared with T98G cells, also from GBM, in order to highlight similarities and differences. The experiments demonstrate that CSC neurospheres host viable and metabolically active cells with neuronal markers such as GABA and glial markers such as gln.