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Abstract #3146

A 1H MRS Study on Neurospheres of Cancer Stem Cells from Human Glioblastoma Multiforme Shows the Presence of Markers of Both Glial & Neuronal Morphology

Laura Guidoni1, Lucia Ricci Vitiani2, Simona di Martino3, Sveva Grande1, Anna Maria Luciani1, Alessandra Palma4, Vincenza Viti1, Antonella Rosi1

1Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanit and INFN, Rome, Italy; 2Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanit, Rome, Italy; 3Scuola Superiore di Catania, University of Catania, Catania, Italy; 4Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanit , Rome, Italy

Glioblastoma multiforme (GBM) is the most common and aggressive type of primary brain tumor. Cancer stem cells (CSC) refractory to the usual therapies has been demonstrated for maintenance of a subset of self-renewing cells producing cancer recurrence. We here propose 1H MRS to study the metabolism in two CSC lines from primary GBM. The CSCs grown as neurospheres are characterised and compared with T98G cells, also from GBM, in order to highlight similarities and differences. The experiments demonstrate that CSC neurospheres host viable and metabolically active cells with neuronal markers such as GABA and glial markers such as gln.