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Abstract #4262

Changes in Lipid Droplet Composition Detected by 1H MRS During Cisplatin Treatment of DAOY Cells

Xiaoyan Pan1,2, Martin Wilson1,2, Carmel McConville1, Julian L. Griffin3, Theodoros N. Arvanitis2,4, Risto A. Kauppinen5, Andrew C. Peet1,2

1Cancer Sciences, University of Birmingham, Birmingham, United Kingdom; 2Birmingham Childrens Hospital NHS Foundation Trust, Birmingham, United Kingdom; 3Biochemistry, University of Cambridge, Cambridge, United Kingdom; 4School of Electronic, Electrical & Computer Engineering, University of Birmingham, Birmingham, United Kingdom; 5Department of Radiology, Dartmouth College, Hanover, NH, United States

In this study, human medulloblastoma cells were treated with cisplatin and the accumulation of lipid droplets (LDs) was monitored by microscopy during cell kill. 1H MRS was performed on whole cells, the isolated LDs fraction and their extracts to investigate the spectral patterns of these accumulated lipids. The increase in detected lipids in cisplatin exposed DAOY cells was associated with the accumulation of LDs and occurred before the DNA fragmentation. 1H MRS of isolated LDs showed alterations in lipid signals at 5.4ppm and 1.25ppm after drug treatment indicating a shift towards unsaturation of the accumulated lipids.