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Abstract #1712

Ferumoxytol-Enhanced MRI of Macrophages in CHL-1melanoma Tumor Model

Hasan Alsaid1, Tinamarie Skedzielewski1, Bao Hoang2, Mary V. Rambo1, James Tunstead2, Christopher Hopson3, Beat M. Jucker1

1Preclinical & Translational Imaging, LAS, PTS, GlaxoSmithKline, King of Prussia, PA, United States; 2Molecular Discovery Research, PTS, GlaxoSmithKline, King of Prussia, PA, United States; 3Oncology R&D, GlaxoSmithKline, Collegeville, PA, United States

In this study, we investigated the optimal ferumoxytol concentration, MRI detection scheme and timeframe of macrophages in CHL-1 melanoma xenograft tumor bearing mice. The results suggest that ferumoxytol-enhanced MRI can be used to detect macrophages in CHL-1 melanoma cell derived xenograft tumors. Ferumoxytol was washed out the tumor after one week of 0.5 mmol Fe/kg injection, reflecting the potential to use ferumoxytol-enhanced MRI on a weekly basis to serially monitor response to drug therapies aimed at increasing cellular phagocytosis. Finally, the lower dose of ferumoxytol may be optimal as there will be greater potential to detect increased cellular iron uptake by scavenging macrophage in the tumor.