Samata Kakkad1, 2, Alireza Akhbardeh1, Jiangyang Zhang3, Meiyappan Solaiyappan1, Dieter Leibfritz2, Kristine Glunde4, Zaver M. Bhujwalla4
1JHU ICMIC Program, Division of Cancer Imaging Research, The Johns Hopkins University SOM, Baltimore, MD, United States; 2Department of Chemistry and Biology, University of Bremen, Bremen, Germany; 3Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University SOM, Baltimore, MD, United States; 4JHU ICMIC Program, Division of Cancer Imaging Research, Johns Hopkins University, Baltimore, MD, United States
Collagen 1 fibers are a major component of the tumor extracellular matrix. Col1 fibers play an important role in macromolecular transport and cancer cell dissemination. Our goal was to determine the influence of Col1 fiber distribution on water diffusion in human breast cancer. We have combined high-resolution diffusion tensor imaging of human breast cancer biopsy followed by second harmonic generation microscopy to intrinsically detect Col1 fibers. Using Haralick texture analysis we classified Col1 fiber distribution feature and observed high water diffusion and diffusion anisotropy correlated with regions of high Col1 fibers. Noninvasive DTI may be used to access Col1 fiber architecture.