Jeroen A. Jeneson1, Ranjan Dash2, Daniel A. Beard2, Robert W. Wiseman3
1Center for Systems Biology of Energy Metabolism and Ageing, University Medical Center Groningen, Groningen, Netherlands; 2Physiology, Medical College of Wisconsin, Milwaukee, WI, United States; 3Physiology and Radiology, Michigan State University, East Lansing, MI, United States
Dynamic 31P NMR spectroscopy has longtime been used to probe the homeostatic performance of the integrated biochemical networks involved in cellular energy balance in a variety of human diseases including heart failure and diabetes. Recent advances in computational modeling of these biochemical networks now offer a tool to link these macroscopic 31P MRS observations to the rich knowledge base on the molecular components interacting in these networks. Here, we explore the potential of such kinetic model-based analysis of dynamic 31P MRS recordings of ATP metabolism in muscle to extract information on the activity of a key mitochondrial enzyme, Pyruvate Dehydrogenase.