Abstract #0477
Detection of transgene expression using hyperpolarized 13 C urea and diffusion-weighted MRS
P. Stephen Patrick 1 , Mikko I. Kettunen 1,2 , Sui-Seng Tee 1 , Tiago B. Rodrigues 1 , Eva M. Serrao 1 , Kerstin Timm 1 , and Kevin M. Brindle 1
1
CRUK Cambridge Institute and Department of
Biochemistry, University of Cambridge, Cambridge,
Cambridgeshire, United Kingdom,
2
A.I.Virtanen
Institute for Molecular Sciences, University of Eastern
Finland, Kuopio, Finland
Diffusion spectroscopy of hyperpolarized [
13
C]urea
was used to assess urea transporter expression as a
potential gene reporter
in
vivo
. Significantly decreased apparent diffusion
coefficient was measured for urea transporter expressing
tumors compared to their non-expressing controls. No
differences in tumor cellularity were observed. The
results suggest that expression of transporter in
combination with diffusion spectroscopy might be used as
another approach for detecting gene reporter expression.
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