Abstract #0683
Developing a nanobiosensor for non-invasive visualization of the AKT signaling pathways
Amnon Bar-Shir 1,2 , Nikita Oskolkov 1,3 , Galit Pelled 1,3 , Jeff W.M. Bulte 1,2 , Michael T McMahon 1,3 , and Assaf A. Gilad 1,2
1
Radiology, Johns Hopkins University,
Baltimore, MD, United States,
2
Institute
for Cell Engineering, Johns Hopkins University,
Baltimore, MD, United States,
3
F.M.
Kirby Research Center, Kennedy Krieger Institute,
Baltimore, MD, United States
The goal of this study is to develop a non-invasive
platform to visualize the response to protein kinase
inhibitors in brain cancer. For that end, we started
with screening for an ideal peptide that will provide
high CEST contrast that will be reduced only upon
phosphorylation by the enzyme AKT. Three peptides,
substrates of the enzyme AKT, generated CEST contrast.
Adding phosphorus to the threonine residue resulted in
reduction of the contrast. Thus, these finidngs are the
first step in optimizing a peptide with the best
senstivity and specificity toward AKT.
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