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Abstract #0683

Developing a nanobiosensor for non-invasive visualization of the AKT signaling pathways

Amnon Bar-Shir 1,2 , Nikita Oskolkov 1,3 , Galit Pelled 1,3 , Jeff W.M. Bulte 1,2 , Michael T McMahon 1,3 , and Assaf A. Gilad 1,2

1 Radiology, Johns Hopkins University, Baltimore, MD, United States, 2 Institute for Cell Engineering, Johns Hopkins University, Baltimore, MD, United States, 3 F.M. Kirby Research Center, Kennedy Krieger Institute, Baltimore, MD, United States

The goal of this study is to develop a non-invasive platform to visualize the response to protein kinase inhibitors in brain cancer. For that end, we started with screening for an ideal peptide that will provide high CEST contrast that will be reduced only upon phosphorylation by the enzyme AKT. Three peptides, substrates of the enzyme AKT, generated CEST contrast. Adding phosphorus to the threonine residue resulted in reduction of the contrast. Thus, these finidngs are the first step in optimizing a peptide with the best senstivity and specificity toward AKT.

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