Overhauser-enhanced MRI as a non invasive probe of BBB breakdown and redox state following ischemia/reperfusion
Matthew S. Rosen 1,2 , Mathieu Sarracanie 1,2 , Brandon D. Armstrong 1,2 , Fanny Herisson 3,4 , Najat Salameh 1,5 , and Cenk Ayata 3,4
Department of Radiology, MGH/Athinoula A.
Martinos Center for Biomedical Imaging, Charlestown, MA,
of Physics, Harvard University, Cambridge, MA, United
of Radiology, MGH/Neurovascular Research Lab,
Charlestown, MA, United States,
of Radiology, Harvard Medical School, Boston, MA, United
de Physique des Systmes Biologiques, EPFL, Lausanne,
A new method to probe hyperacute BBB breakdown following
ischemic stroke in a rodent model using Overhauser-MRI (OMRI)
in conjunction with an injected stable free radical,
TEMPOL, is described. We present OMRI images of TEMPOL
crossing the BBB following ischemia/reperfusion in vivo.
The use of OMRI in conjunction with TEMPOL as an
exogenous imaging agent for stroke is a new and novel
approach, and suggests that TEMPOL may be a suitable
probe for observing early BBB breakdown following
reperfu-sion in rodent I/R models. We hypothesize that
temporally resolved OMRI may be used to indicate the
redox status of ischemic tissue.
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