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Abstract #0986

Reduction of (1- 13 C)-dehydroascorbic acid to (1- 13 C)-ascorbic acid is not correlated to glutathione in a treatment response model of murine lymphoma in vivo

Kerstin N Timm 1,2 , Mikko I Kettunen 1,2 , De E Hu 1,2 , Tiago B Rodrigues 1,2 , Timothy J Larkin 1,2 , Irene Marco-Rius 1,2 , and Kevin M Brindle 1,2

1 Department of Biochemistry, University of Cambridge, Cambridge, Cambridgshire, United Kingdom, 2 Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, Cambridgshire, United Kingdom

Hyperpolarized [1-13C]-dehydroascorbic acid (DHA), the oxidized form of vitamin C, can be used as a magnetic resonance marker of redox state in vitro and in vivo. What limits the reduction of hyperpolarized [1-13C]-DHA to [1-13C]-ascorbic acid (AA) in vivo and hence which metabolic process it directly reports on is still poorly understood. We treated EL4 tumor bearing mice with the topoisomerase inhibitor etoposide and showed that the reduction rate of hyperpolarized [1-13C]-DHA in vivo is highly variable, which was not correlated with intracellular glutathione levels. This suggests contribution of other factors such as NADPH from the pentose phosphate pathway.

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