Metabolic Analysis of Slowly Cycling Melanoma Cells
Sergey Magnitsky 1 and Geetha Mohan 1
Radiology and Biomedical Imaging, University
of California San Francisco, San Francisco, California,
Low efficacy of existing treatment for various human
cancers has been attributed to the existence of cancer
stem cells. Such slowly cycling sub-population of cells
in melanoma exhibits high tumorigenicity, self-renewal
capacity and drug resistance. The goal of this study was
to delineate metabolic differences between slowly
cycling sub-population and bulk tumor cells.
Ethanol/chloroform cells extracts were prepared from
sorted cells. High-resolution NMR spectra were acquired.
NMR data revealed high intracellular lactate and choline
concentration in slowly cycling sub-population cells
compared to fast cycling tumor cells. Approximately 70%
of lactate produced in unsorted cells was produced by
slowly cycling sub-population.
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