Metabolic Analysis of Slowly Cycling Melanoma Cells

Low efficacy of existing treatment for various human cancers has been attributed to the existence of cancer stem cells. Such slowly cycling sub-population of cells in melanoma exhibits high tumorigenicity, self-renewal capacity and drug resistance. The goal of this study was to delineate metabolic differences between slowly cycling sub-population and bulk tumor cells. Ethanol/chloroform cells extracts were prepared from sorted cells. High-resolution NMR spectra were acquired. NMR data revealed high intracellular lactate and choline concentration in slowly cycling sub-population cells compared to fast cycling tumor cells. Approximately 70% of lactate produced in unsorted cells was produced by slowly cycling sub-population.

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