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Abstract #1086

Role of Choline Kinase and Ethanolamine Kinase Isoforms in Modulating Phosphoethanolamine Levels in Breast Cancer Cells

Tariq Shah 1 , Balaji Krishnamachary 1 , Flonne Wildes 1 , Jannie Wijnen 2 , Kristine Glunde 1 , and Zaver M Bhujwalla 1

1 Division of Cancer Imaging Research, Department of Radiology, Johns Hopkins University, Baltimore, Maryland, United States, 2 Cancer center, University Medical Centre Utrecht, Utrecht, Netherlands

While significant effort has been focused on the increased phosphocholine (PC) levels observed in cancer cells and tumors, increased phosphoethanolamine (PE) has been underexplored. Increased PC, but not PE, is observed in cells in culture because culture medium contains free choline, but no ethanolamine. We have used siRNAs to silence specific genes involved in choline and ethanolamine metabolism to understand their roles in intracellular metabolite levels measured with high-resolution phosphorus MR spectroscopy of cell extracts. We have demonstrated that both Chk-α and EthnK1 contribute to PE levels observed in vivo with the latter having a primary role in its biosynthesis.

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