Abstract #1086
Role of Choline Kinase and Ethanolamine Kinase Isoforms in Modulating Phosphoethanolamine Levels in Breast Cancer Cells
Tariq Shah 1 , Balaji Krishnamachary 1 , Flonne Wildes 1 , Jannie Wijnen 2 , Kristine Glunde 1 , and Zaver M Bhujwalla 1
1
Division of Cancer Imaging Research,
Department of Radiology, Johns Hopkins University,
Baltimore, Maryland, United States,
2
Cancer
center, University Medical Centre Utrecht, Utrecht,
Netherlands
While significant effort has been focused on the
increased phosphocholine (PC) levels observed in cancer
cells and tumors, increased phosphoethanolamine (PE) has
been underexplored. Increased PC, but not PE, is
observed in cells in culture because culture medium
contains free choline, but no ethanolamine. We have used
siRNAs to silence specific genes involved in choline and
ethanolamine metabolism to understand their roles in
intracellular metabolite levels measured with
high-resolution phosphorus MR spectroscopy of cell
extracts. We have demonstrated that both Chk-α and
EthnK1 contribute to PE levels observed in vivo with the
latter having a primary role in its biosynthesis.
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