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Abstract #1087

CEST imaging of human breast cancer cells

Catherine DeBrosse 1 , Mohammad Haris 1 , Ravi Prakash Reddy Nanga 1 , Hari Hariharan 1 , Damodar Reddy 1 , Imran Mohammad 2 , Kejia Cai 1 , Anup Singh 1 , and Ravinder Reddy 1

1 Center for Magnetic Resonance and Optical Imaging, Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States, 2 Department of Pharmacology and Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania, United States

The purpose of this study was to determine feasibility of glutamate chemical exchange saturation transfer (GluCEST) imaging in human breast cancer cell lines treated with poly-L-glutamate (PLG). PLG has been used as a drug-conjugate in recently developed cancer therapies, as it is broken down by tumor proteases. Glutamate fragments that result from PLG degradation have labile amine protons that exchange with water and give GluCEST signal. In this study, we demonstrate that treatment of human breast cancer cells with PLG led to an increase in GluCEST signal due to the breakdown of PLG by tumor proteases such as cathepsins.

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