Abstract #1088
The effect of aminooxyacetate on metabolism of breast cancer cells
Noriko Mori 1 , Preethi Korangath 2 , Santosh Bharti 1 , Flonn Wildes 1 , Saraswati Sukumar 1,2 , and Zaver M. Bhujwalla 1,2
1
The Russell H. Morgan Department of
Radiology and Radiological Science, The Johns Hopkins
University School of Medicine, Baltimore, Maryland,
United States,
2
The
Sidney Kimmel Comprehensive Cancer Center, The Johns
Hopkins University School of Medicine, Baltimore,
Maryland, United States
Aminooxyacetate (AOA) is a known inhibitor of the
transamination reaction. We previously found that AOA
treatment showed dose dependent growth inhibitory
activity in breast cancer cells, especially in glutamine
dependent cancer cells such as SUM159. To investigate
the mechanism of AOA action, we used 1H MRS of cell
extracts and conditioned media from SUM159 and observed
reductions of alanine, aspartate, phosphocholine, and
increases of glutamate and tyrosine following treatment
with 2mM AOA for 24h. Lactate production and glucose
consumption in conditioned media and the level of Chk-α
that catalyzes the formation of phosphocholine were not
affected by AOA.
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