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Abstract #1418

Effect of embedding media on post-mortem MRI of formalin-fixed brain tissue at 7.0 T

Petr Dusek 1,2 , Vince Istvan Madai 3 , Matthias Dieringer 4,5 , Fabian Hezel 4 , Thoralf Niendorf 4,5 , Jan Sobesky 3,5 , Radoslav Matej 6 , and Jens Wuerfel 1,7

1 Institute of Neuroradiology, University Medicine Goettingen, Goettingen, Germany, 2 Department of Neurology and Center of Clinical Neuroscience, Charles University in Prague, 1st Faculty of Medicine and General University Hospital in Prague, Praha, Czech Republic, 3 Department of Neurology and Center for Stroke Research Berlin (CSB), Charit-Universitaetsmedizin, Berlin, Germany, 4 Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine, Berlin, Germany, 5 Experimental and Clinical Research Center (ECRC), Charit-Universitaetsmedizin and Max Delbrueck Center for Molecular Medicine (MDC), Berlin, Germany, 6 Department of Pathology and Molecular Medicine, Thomayer Teaching Hospital, Praha, Czech Republic, 7 NeuroCure Clinical Research Center, Charit-Universitaetsmedizin, Berlin, Germany

It is well known that formalin fixation of post-mortem material affects its contrast mechanism and MR characteristics. It is however not clear how these parameters and image quality are affected by the medium in which is the post-mortem material embedded during scanning. Our goal was to compare different embedding media (deuterium oxide (D2O), formalin, low-melting temperature agarose and phosphate-buffered-saline) for post-mortem MRI and ascertain which gives the best signal-to-noise ratio and contrast results. Also, we wanted to find out whether embedding medium has influence on signal intensity of formalin fixed brain slices. We employed T2 mapping, T1 mapping, T2* mapping, MP-RAGE and TIRM sequences. The results for all embedding media are shown.

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