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Abstract #2769

Hyperpolarized Ketone Body Metabolism in the Perfused Rat Heart

Damian J Tyler 1 , Angus Z Lau 1,2 , and Daniel R Ball 1

1 Dept. of Physiology, Anatomy & Genetics, University of Oxford, Oxford, Oxfordshire, United Kingdom, 2 Dept. of Cardiovascular Medicine, University of Oxford, Oxford, Oxfordshire, United Kingdom

Hyperpolarization of [1- 13 C]pyruvate provides a unique insight into various aspects of carbohydrate metabolism. However, alterations in the metabolic pathways of other key fuel molecules (e.g. fatty acids and ketone bodies) are a common feature of many diseases. This work aimed to produce a reliable method to hyperpolarize the ketone bodies, acetoacetate and β-hydroxybutyrate, and to investigate their metabolism in the perfused rat heart. Following successful polarization of both substrates, perfusion into the isolated rat heart enabled direct visualization of the interconversion between the ketone bodies along with observation of their downstream metabolism in the Krebs cycle.

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