Abstract #2793
Detection of metabolic changes in SCC tumor by mTOR inhibition using hyperpolarized 13 C-pyruvate MRI
Keita Saito 1 , Shingo Matsumoto 1 , Yoichi Takakusagi 1 , Masayuki Matsuo 1 , H Douglas Morris 2 , Hellmut Merkle 2 , Martin J Lizak 2 , Jeeva P Munasinghe 2 , Nallathamby Devasahayam 1 , Sankaran Subramanian 1 , James B Mitchell 1 , and Murali C Krishna 1
1
Radiation Biology Branch, National Cancer
Institute, Bethesda, Maryland, United States,
2
National
Institute of Neurological Disorder and Stroke, Bethesda,
Maryland, United States
We investigated effects of an mTOR inhibitor rapamycin
on pyruvate metabolism in squamous cell carcinoma (SCC)
implanted in mice leg using
13
C-MRI
with hyperpolarized [1-
13
C]pyruvate. The
signal of [1-
13
C]pyruvate and [1-
13
C]lactate
were detected in SCC tumor immediately after
hyperpolarized [1-
13
C]pyruvate injection. [1-
13
C]lactate
to [1-
13
C]pyruvate ratio (Lac/Pyr) in the SCC
tumors increased as tumor grew in non-treated control
mice, whereas it significantly dropped after 2 days of
rapamycin treatment. So, mTOR inhibition causes decrease
of LDH activity in SCC tumor, and lactate to pyruvate
ratio monitored using hyperpolarized
13
C-MRI
would become a useful marker for tumor response to
therapy.
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