Abstract #2863

Peptide-based MRI contrast agent and near-infrared ﬂuorescent probe for intratumoral legumain detection

Gin-Chung Liu ¹ , Yu-Jen Chen ² , Chia-Yun Chen ¹ , Shou-Cheng Wu ² , and Yun-Ming Wang ²

¹ Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, ² Department of Biological Science and Technology, National Chiao Tung University, HsinChu, Taiwan

In this study, we developed a MRI contrast agent ([Gd-NBCB-TTDA-Leg(L)]) and a NIR fluorescence (NIRF) probe (CyTE777-Leg(L)-CyTE807) to monitor legumain activity in tumors. The MRI contrast agent was prepared by introduction of cyclobutyl and benzyl group residues to TTDA, followed by a legumain-specific substrate peptide (Leg(L)). The NIRF probe was designed by conjugating two fluorochromes with Leg(L). Peptide cleavage of the MRI contrast agent by legumain can increase its hydrophobicity and promote contrast enhancements. Similar cleavage effect on NIR probes relieves the self quench of the probe.

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