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Abstract #3764

The Intracellular Water Preexchange Lifetime of Neurons and Astrocytes Are Different and Decrease Rapidly under Oxygen-Glucose-Deprivation Conditions

Donghan Yang 1 , James E Huettner 2 , Jeffrey J Neil 3,4 , and Joseph J Ackerman 1,5

1 Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri, United States, 2 Department of Cell Biology & Physiology, Washington University in St. Louis, St. Louis, Missouri, United States, 3 Department of Neurology, Washington University in St. Louis, St. Louis, Missouri, United States, 4 Department of Pediatrics, Washington University in St. Louis, St. Louis, Missouri, United States, 5 Department of Radiology, Washington University in St. Louis, St. Louis, Missouri, United States

The intracellular water preexchange lifetime ( lower case Greek tau IN ) determines in what exchange regime diffusion-weighted MR data should be interpreted. We have determined lower case Greek tau IN for microbead-adherent cultures of rat cerebral cortical neurons and astrocytes: 0.88 plus-or-minus sign 0.24 s for neurons and 0.66 plus-or-minus sign 0.17 s for astrocytes. Upon oxygen-glucose-deprivation, a rapid lower case Greek tau IN decrease was observed: 0.48 plus-or-minus sign 0.07 s and 0.18 plus-or-minus sign 0.04 s, respectively. Diffusion times used in clinical studies of normal subjects place them in the slow-exchange regime. The non-negligible difference between neuron and astrocyte lower case Greek tau IN , especially in injured cells, should be taken into account in the analysis of time-scale-sensitive data.

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