Abstract #3814
Feasibility of accurately determining cell number by 19 F MRI and the impact of cellular rejection, inflammation and transfer of label
Jeff M Gaudet 1,2 , Emeline J Ribot 3 , Yuhua Chen 1 , Kyle Gilbert 4 , and Paula Foster 1,2
1
Imaging Research Laboratories, Robarts
Research Institute, London, Ontario, Canada,
2
Medical
Biophysics, University of Western Ontario, London,
Ontario, Canada,
3
Centre
de Resonance Magnetique des Systemes Biologiques,
Universite Bordeaux, France,
4
Centre
for Functional and Metabolic Mapping, Robarts Research
Institute, London, Ontario, Canada
Stem cell therapy has the potential to revolutionize
modern medicine and clinical trials are already
underway. Still, questions persist concerning which
parameters (implantation method, size of transplant,
timing) produce the best clinical outcomes. Fluorine-19
(
19
F) MRI provides an excellent tool to
address these issues because of the potential for
unambiguous detection and accurate quantification. In
this study we correlate the change in
19
F-signal
quantification over time with fluorescence microscopy
and immunohistochemistry. Our results indicate that
accurate quantification of the cellular number is
possible under some conditions and that
19
F
labeling of bystander macrophages can confuse
interpretation in certain cases.
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