Method of transforming brain spectroscopic waterline data into standard brain space (analyzing functional MRS in FSL)

We present a method to register waterline spectroscopic brain data to standard MNI brain for inter-subject analysis. High spatial resolution non-water suppressed 3D EPSI data with CSF suppression inversion recovery pulse was acquired from visual cortex during a flickering checkerboard visual presentation. Principal component analysis was performed on the resulting data. 1st PC score map was registered to high spatial resolution structural MPRAGE. The transformation matrix of this registration was applied to the 2nd PC score map. MPRAGE was registered to standard brain and its transformation matrix was applied to 1st or 2nd PC score maps to enable group analysis.

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