Assessment of hepatic glycogen turnover in mice by in vivo 13 C-MRS

We investigated the feasibility to assess hepatic glycogen turnover in mice by in vivo ¹³ C-MRS. For this purpose, mice were infused with [1- ¹³ C]-labeled glucose (N=2), or galactose (N=3) for 120min, followed by 120min of infusion with the respective unlabeled substrate, while 13C-MR spectra of the liver were recorded throughout the experiment. Glycogen-signals increased in the first, and decreased in the second phase of all experiments indicating simultaneous synthesis and degradation. Turnover (rate of degradation/rate of synthesis) was determined from fitting the kinetics of glycogen to a metabolic model and was estimated to be ~84% with galactose as tracer substrate.

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