Meeting Banner
Abstract #0206

Assessment of hepatic glycogen turnover in mice by in vivo 13 C-MRS

Andreas Boss 1 , Andor Veltien 1 , and Arend Heerschap 1

1 Radiology and Nuclear Medicine, Radboudumc, Nijmegen, Gelderland, Netherlands

We investigated the feasibility to assess hepatic glycogen turnover in mice by in vivo 13 C-MRS. For this purpose, mice were infused with [1- 13 C]-labeled glucose (N=2), or galactose (N=3) for 120min, followed by 120min of infusion with the respective unlabeled substrate, while 13C-MR spectra of the liver were recorded throughout the experiment. Glycogen-signals increased in the first, and decreased in the second phase of all experiments indicating simultaneous synthesis and degradation. Turnover (rate of degradation/rate of synthesis) was determined from fitting the kinetics of glycogen to a metabolic model and was estimated to be ~84% with galactose as tracer substrate.

This abstract and the presentation materials are available to members only; a login is required.

Join Here