Mitochondrial NADH in vivo: functional test reveals a natural indicator of oxidative phosphorylation in 31P spectrum.
Kevin E Conley 1 , Amir Ali 1 , and Sharon Jubrias 1
Radiology, University of Washington,
Seattle, WA, United States
Nicotinamide adenine dinucleotide (NAD) compartmentation
underlies the role of this co-enzyme as a co-factor in
oxidative phosphorylation, glycolysis and mitochondrial
biogenesis. Here we identify a unique resonance for
mitochondrial NADH by phosphorus magnetic resonance
spectroscopy (31P MRS) in vivo. This mitochondrial NADH
pool is identified by chemical standards and dynamic
changes in the NAD+ and NADH peaks with activation of
oxidative phosphorylation in human muscle. The end
result is non-invasive detection of NADH in cell vs.
mitochondria, which provides sensitive natural
indicators of redox compartmentation and oxidative
phosphorylation in human tissues in vivo.
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