Meeting Banner
Abstract #0319

Resonance frequency-shifting nitroxide for probing proteolytic activity in vivo using the Overhauser-enhanced MRI technique

Neha KOONJOO 1 , Grard Audran 2 , Lionel Bosco 2 , Paul Brmond 2 , Elodie Parzy 1 , Philippe Massot 1 , Matthieu Lepetit-Coiff 1,3 , Jean-Michel Franconi 1 , Sylvain R.A Marque 2 , Eric Thiaudire 1 , and Philippe Mellet 1,4

1 Centre de Rsonance Magntique des Systmes Biologiques, Bordeaux, France, Metropolitan, 2 UMR 7273 Aix-Marseille Universit, Marseille, France, Metropolitan, 3 Siemens, Saint-Denis, France, Metropolitan, 4 INSERM, Universit de Bordeaux Segalen, Bordeaux, France, Metropolitan

Proteolysis of a line-shifting nitroxide-OMRI probe was visualized in the digestive tract of living mice using Overhauser-enhanced MRI. Upon enzymatic action on the probe, its hyperfine coupling constants are shifted; thus resulting in two detectable electronic excitation frequencies enabling both substrate and end product visualization. High signal enhancements revealed either the substrate or its end product in the stomach. Highly resolved 3D-keyhole OMRI images of resolution 0.5mm 3 were obtained with a fully balanced steady state sequence TrueFISP at 0.2T in 18 seconds. This work is a steppingstone in in vivo pathology-related proteolysis detection.

This abstract and the presentation materials are available to members only; a login is required.

Join Here