Abstract #0327
Metabolic flux analysis of hepatic mitochondrial oxidation of hyperpolarized [1- 13 C] and [2- 13 C] pyruvate in vivo
Emine Can 1 , Jessica A.M. Bastiaansen 2,3 , Hikari A.I. Yoshihara 1,4 , Rolf Gruetter 5,6 , and Arnaud Comment 1
1
Institute of Physics of Biological Systems,
EPFL, Lausanne, Switzerland,
2
Department
of Radiology, University Hospital Lausanne (CHUV) and
University of Lausanne (UNIL), Lausanne, Switzerland,
3
Center
for Biomedical Imaging (CIBM), Lausanne, Switzerland,
4
Department
of Cardiology, University Hospital Lausanne (CHUV),
Lausanne, Switzerland,
5
Laboratory
for Functional and Metabolic Imaging, EPFL, Lausanne,
Switzerland,
6
Department
of Radiology, University of Lausanne, University of
Geneva, Switzerland
Hepatic 13C MRS studies are challenging due to abundant
intracellular lipid resonances, impairing the detection
of 13C glutamate labeling commonly used to measure TCA
cycle fluxes. The carboxyl resonances typically detected
by hyperpolarized 13C MRS do not interfere with lipid
resonances. In this study, we assessed hepatic
metabolism in vivo in real time using hyperpolarized
[2-13C]pyruvate and [1-13C]pyruvate to detect the
contributions to mitochondrial metabolism related to
pyruvate carboxylase and pyruvate dehydrogenase
activities. Using the 13C labeling of TCA cycle
intermediates within a single 1-min experiment, TCA
cycle fluxes were estimated, enabling comparative
studies of different metabolic states.
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