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Abstract #0327

Metabolic flux analysis of hepatic mitochondrial oxidation of hyperpolarized [1- 13 C] and [2- 13 C] pyruvate in vivo

Emine Can 1 , Jessica A.M. Bastiaansen 2,3 , Hikari A.I. Yoshihara 1,4 , Rolf Gruetter 5,6 , and Arnaud Comment 1

1 Institute of Physics of Biological Systems, EPFL, Lausanne, Switzerland, 2 Department of Radiology, University Hospital Lausanne (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland, 3 Center for Biomedical Imaging (CIBM), Lausanne, Switzerland, 4 Department of Cardiology, University Hospital Lausanne (CHUV), Lausanne, Switzerland, 5 Laboratory for Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, 6 Department of Radiology, University of Lausanne, University of Geneva, Switzerland

Hepatic 13C MRS studies are challenging due to abundant intracellular lipid resonances, impairing the detection of 13C glutamate labeling commonly used to measure TCA cycle fluxes. The carboxyl resonances typically detected by hyperpolarized 13C MRS do not interfere with lipid resonances. In this study, we assessed hepatic metabolism in vivo in real time using hyperpolarized [2-13C]pyruvate and [1-13C]pyruvate to detect the contributions to mitochondrial metabolism related to pyruvate carboxylase and pyruvate dehydrogenase activities. Using the 13C labeling of TCA cycle intermediates within a single 1-min experiment, TCA cycle fluxes were estimated, enabling comparative studies of different metabolic states.

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