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Abstract #0328

Detection of lung mitochondrial dysfunction using hyperpolarized [1-13C] pyruvate metabolism

Hoora Shaghaghi 1 , Stephen Kadlecek 1 , Mehrdad Pourfathi 1 , Sarmad Siddiqui 1 , Maurizio Cereda 2 , Hooman Hamedani 1 , Harrilla Profka 1 , Yi Xin 1 , and Rahim R. Rizi 1

1 Radiology, University of Pennsylvania, Philadelphia, PA, United States, 2 Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA, United States

Mitochondrial dysfunction is associated with various forms of lung injury and disease. To address the effect of mitochondrial dysfunction on lung metabolism, HP-Pyruvate metabolism was studied in presence of complex I inhibitor (rotenone) and complex IV activator (TMPD). Rotenone decreased bicarbonate production significantly (49%) and TMPD elevated it about 74% when compared to control. Lactate label exchange significantly increased in treated lungs with rotenone (53%). Enhancement of lactate production of injured lungs could be from presence of inflammatory cells and/or because of mitochondrial dysfunction. But the bicarbonate production is not changed in inflamed lungs and decreased on dysfunction of mitochondria.

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