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Abstract #0415

MEMRI of organotypic rat hippocampal slice cultures

Alexia Daoust 1 , Stephen Dodd 1 , and Alan Koretsky 1

1 NINDS, LFMI, NIH, Bethesda, MD, United States

MEMRI can be used for different applications such as tracing neuronal connections. However, the detailed mechanisms responsible for Mn transport and synaptic transmission are still unclear. To study these mechanisms, a hippocampal slice culture was developed that enables high resolution MEMRI. Excellent MRI contrast of hippocampal structures could be obtained after incubating slices with 10 uM for 24 hours. Mn microinjection into CA3 led to tract tracing into CA1/2 and then in the Schaffer collaterals. This hippocampal slice culture should be useful to study mechanisms of Mn transport in neurons and across synapses.

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