Meeting Banner
Abstract #0424

In Vivo Assessment of Intracellular NAD + /NADH Redox State in Human Brain at 4 Tesla

Ming Lu 1 , Wei Chen 1 , and Xiao-Hong Zhu 1

1 Center for Magnetic Resonance Research, University of Minnesota Medical School, Minneapolis, Minnesota, United States

NAD + and NADH play key roles in cellular respiration. Intracellular redox state defined by the NAD + /NADH ratio (RX) reflects the cellular metabolic and physiopathological status. By taking the advantage of high/ultrahigh magnetic field strengths, we have recently established a novel in vivo 31 P MRS based NAD assay for noninvasive measurements of intracellular NAD concentrations and redox state in animal and human brains at 16.4 T, 9.4 T and 7 T, respectively. To explore its potential for clinical translation, in this study, we investigated the feasibility of assessing the NAD metabolism and redox state in human brain at a relatively lower field of 4 T by incorporating 1 H-decoupling technique with the 31 P NAD assay. The use of 1 H-decoupling significantly narrowed the linewidths of NAD and lower case Greek alpha -ATP resonances, resulting in higher sensitivity and better-resolved resonance signals as compare to the 1 H-coupled spectrum. These improvements made it possible for reliably quantifying cerebral RX and NAD concentrations, which are consistent with previously reported results obtained at 7 T in similar aged human subjects. In summary, this work demonstrates the capability and utility of the 1 H-decoupled 31 P MRS-based NAD assay at lower field strength, which indicates potential opportunities for studying NAD metabolism and redox state in human brain at clinical setting.

This abstract and the presentation materials are available to members only; a login is required.

Join Here